Fig. 7

Conditional microglia BAP31 knockout mice exhibit more inflammation when administered LPS. a Schematic representation of the breeding strategy. The representation of the breeding strategy. b PCR analysis of BAP31^fl/fl, BAP31^wt/wt, and Cre genes in the second offspring. c Immunocytochemistry for BAP31 (green) and Iba1 (red) was performed in the primary microglial cells of the WT and KO mice. Scale bar = 50 μm. d The relative immunofluorescence intensity was used to represent the protein levels of BAP31 and Iba1. e Western blot analysis of BAP31and Iba1 in primary microglia of the WT and KO mice. f–i Levels of IL-1β, TNFα, iNOS, and COX2 mRNA in samples of the hippocampus were analyzed by RT-PCR. Primary WT and KO microglial cells were treated with LPS (100 ng/ml) for 24 h. The protein levels of the cytokines IL-1β (j) and TNFα (k) in cells were analyzed by Western bloting; all the data are indicated as Mean ± SEM of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 versus control group