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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Clemastine improves hypomyelination in rats with hypoxic–ischemic brain injury by reducing microglia-derived IL-1β via P38 signaling pathway

Fig. 2

Histamine H1 receptor (HH1R) protein expression in the corpus callosum of rats at 1, 3, and 7 days after the bilateral common carotid artery occlusion (BCCAO) and clemastine injection after BCCAO when compared with their corresponding controls. Double immunofluorescence staining showing the distribution of Iba-1-labeled (a, d, and g, green) and HH1R (B, E, and H, red) immunoreactive amoeboid microglial cells (AMCs) in the corpus callosum of rats at 1 day after BCCAO and clemastine injection after BCCAO and their corresponding controls. The colocalized expression of Iba-1 and HH1R in AMCs can be seen in c, f, and i. Panel j shows HH1R (70 kDa) and β-actin (42 kDa) immunoreactive bands. Panels K and L show bar graphs depicting significant increases in the immunofluorescence intensity and optical density of HH1R following the BCCAO when compared with their corresponding controls; clemastine showed no effect on the expression of HH1R. N = 3. *P < 0.05. Scale bars: A–I 50 μm.

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Journal of Neuroinflammation

ISSN: 1742-2094