Fig. 5

Clemastine promotes differentiation of oligodendrocyte progenitor cells (OPCs) insulted by IL-1β in vitro. Immunofluorescence images of cultured OPCs show the ratios of NG2 (a–c, green) and MBP (d–f, red) at 7 days after IL-1β administration (b, e) or IL-1β + clemastine treatment (c, f) when compared with their corresponding controls (a, d). Bar graph in g and h shows a remarkable increase in the ratio of NG2-positive oligodendrocytes and decrease in the ratio of MBP-positive oligodendrocytes at 7 days after IL-1β administration when compared with their corresponding controls; clemastine reversed these changes. Panel i shows MBP (32 kDa), CNPase (48 kDa), and β-actin (42 kDa) immunoreactive bands. Bar graphs in j and k show significant decreases in the optical density of MBP and CNPase at 7 days after IL-1β administration when compared with their corresponding controls. The clemastine may attenuate the increment. N = 3. *P < 0.05. Scale bars: a–f 50 μm