Fig. 5

Differentially expressed cytokines and chemokines detected by multiplex protein analysis in the 3-week old mouse cerebella across five genotypes. Progressive loss of functional App allele in NPC mouse model (Npc1^−/−/App^+/− and Npc1^−/−/App^−/−) resulted in significant increase of pro-inflammatory cytokines at 3 weeks of age. Cytokines were measured by multiplexed magnetic bead-based immunoassay kit (Catalog# MCYTMAG-70 K-PX32, Millipore Sigma, Burlington MA). a IFN-γ-responsive cytokine IP-10/CXCL10 is the only protein significantly increased in Npc1^−/−/App^+/+ in the pre-symptomatic mouse cerebella. This increased expression is significantly exacerbated with the loss of APP function (compare Npc1^−/−/App^+/+ with Npc1^−/−/App^+/− and Npc1^−/−/App^−/−). b–d RANTES/CCL5, EOTAXIN/CCL11, and IL-10 were also significantly increased in Npc1^−/−/App^+/− and/or Npc1^−/−/App^−/− mouse cerebella compared with wild-type (Npc1^+/+/App^+/+) and/or Npc1^−/−/App^+/+. e IL-1β expression was reduced in mice lacking APP function (Npc1^+/+/App^−/−). Values are means ± SEM. *p < 0.05, **p < 0.01. * = compared to Npc1^+/+/App^+/+; ^ = compared with Npc1^+/+/App^−/−; # = compared with Npc1^−/−/App^+/+