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Fig. 10 | Journal of Neuroinflammation

Fig. 10

From: Palmitoylethanolamide counteracts substance P-induced mast cell activation in vitro by stimulating diacylglycerol lipase activity

Fig. 10

Co-treatment with subeffective concentrations of PEA and 2-AG down-modulates SP-induced histamine release and degranulation in RBL-2H3 cells. a Histamine release by ELISA, b Toluidine blue staining, and c the percent of degranulation were measured after that RBL-2H3 cells were stimulated with SP (10 μM) and treated with PEA (0.1 and 10 μM), 2-AG (0.1 and 1 μM), or PEA (0.1 μM) plus 2-AG (0.1 μM), for 15 min at 37 °C in a 5% CO2 atmosphere. Absorbance was measured at 450 nm (a). Red arrows show degranulated RBL-2H3 cells (b). Each bar (a, c) shows the mean ± SEM. **p < 0.01 and ***p < 0.001 compared with vehicle. °p < 0.05, °°p < 0.01, and °°°p < 0.001 compared with SP

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