Skip to main content
Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Palmitoylethanolamide counteracts substance P-induced mast cell activation in vitro by stimulating diacylglycerol lipase activity

Fig. 3

PEA and JWH133 control SP-induced histamine release in RBL-2H3 cells via a CB2-mediated mechanism. Histamine release by ELISA was performed after that RBL-2H3 cells were stimulated with SP (10 μM) and treated with AM630 (0.1 μM) in the presence or absence of a PEA (10 μM) or b JWH133 (0.1 μM), for 15 min at 37 °C in a 5% CO2 atmosphere. Absorbance was measured at 450 nm. Each bar shows the mean ± SEM. *p < 0.05 and ***p < 0.001 compared with Vehicle. °°°p < 0.001 compared with SP. ≠≠≠p < 0.001 compared with SP + PEA 10 μM. p < 0.01 compared with SP +JWH133 0.1 μM

Back to article page