Fig. 4
PEA and JWH133 down-modulate SP-induced degranulation of RBL-2H3 cells via a CB2-mediated mechanism. Toluidine blue staining was performed to measure the number of degranulated RBL-2H3 cells after that a RBL-2H3 cells were stimulated with SP (10 μM) in the presence and absence of PEA (10 μM), or JWH133 (0.1 μM), for 15 min at 37 °C in a 5% CO2 atmosphere; b SP-stimulated RBL-2H3 cells were treated with AM630 (0.1 μM), in the presence and absence of PEA (10 μM), or JWH133 (0.1 μM), for the indicated time. Red arrows show degranulated RBL-2H3 cells. c Percentage of degranulation. Each bar shows the mean ± SEM. ***p < 0.001 compared with vehicle. °p < 0.05 and °°°p < 0.001 compared with SP. ≠≠≠p < 0.001 compared with SP + PEA 10 μM. §p < 0.05 compared with SP + JWH133 0.1 μM