Fig. 9

OMDM188 blocks PEA down-modulation of SP-induced histamine release and degranulation in RBL-2H3 cells. a Histamine release by ELISA, b Toluidine blue staining, and c the percent of degranulation were measured after that RBL-2H3 cells were stimulated with SP (10 μM) and treated with PEA (10 μM) in the presence or absence of OMDM188 (10 μM) for 15 min at 37 °C in a 5% CO₂ atmosphere. Absorbance was measured at 450 nm (a). Red arrows show degranulated RBL-2H3 cells (b). Each bar (a, c) shows the mean ± SEM. ***p < 0.001 compared with vehicle. °°°p < 0.001 compared with SP. ^≠≠≠p < 0.001 compared with SP + PEA 10 μM