Fig. 3
From: Unique molecular signature in mucolipidosis type IV microglia
Analysis of CCL5 expression in microglia. a Ccl5 Log2 fold change and Log10 (pAdj) in 2-month-old Fabry disease (FD), 7-week-old Niemann-Pick type C1 (NPC1), 3-month-old amyotrophy lateral sclerosis (SOD1), 16-month-old App-Ps1 (AD), and 2-month-old mucolipidsosis type IV (MLIV) microglia isolated from mouse brain b IBA1 and CCL5 double staining in hippocampi. The scale bar is 10 μm. c CCL5 quantification by enzyme-linked immunosorbent assay (ELISA) in mouse serum. Npc1−/− N = 7, MLIV N = 8, FD N = 16, 2-month-old Gaa−/− (Pompe disease) N = 10, 3-month-old Npc2−/− N = 7, Sod1tgG93A N = 4, 2-, 3-, and 6-month-old Cln3−/− N = 4–6. HPβCD/VTS-270-treated animals were subcutaneously injected every other day from day 7 to 49 [24], N = 4 and 5 for control and Npc1−/−, respectively. d CCL5 ELISA from control and MLIV patient’s serum. Ncontrol = 14, NMLIV = 48