Fig. 4

RAGE^−/− attenuate the increased M1 polarization, high BP, and great RSNA in stressed mice. a Examples of original tracings of RSNA, HR, and BP recording in different groups of mice. Quantitative analysis of b MAP, c, d RSNA, e SBP, and f heart rate (HR) values for RAGE^−/− and stressed mice are shown. g Fluorescence images of microglia immunostained for anti-CD86 (M1 marker) in the RVLM from control, stress, and stress + RAGE^−/− mice (scale bar = 50 μm). M1 activation was reduced in RAGE^−/− mice. h Quantitative data on the mean densitometry of CD86 positivities. Data are presented as mean ± SEM. n = 6, *P < 0.05, ANOVA LSD test