Fig. 8

Lysosomal function is impaired in RAGE^−/− mice. a Representative western blot images and b statistical analysis of RAB7 and LAMP1 expression in microglia. c Double immunofluorescence showed colocalization of RAB7 and LC3 which was analyzed by confocal fluorescence microscopy. d The level of colocalization of LC3 (red) and RAB7 (green) was assessed by using the Pearson coefficient (scale bar = 2 μm). e, f Acidation of lysosome was detected by using the Lyso-Tracker/Lyso-Sensor staining (scale bar = 2 μm). Data are presented as mean ± SEM. n = 6, *P < 0.05, ANOVA LSD test