Fig. 4

Ex vivo gene and protein characterization of c-Met⁺ versus c-Met^-CD8⁺ T cells. a Relative expression of EOMES, RUNX3, FOXP3, GATA3, and T-bet mRNA in FACS-sorted spleen cells of peak disease c-Met⁺ CD3⁺ CD8⁺ vs. c-Met⁻ CD3⁺ CD8⁺ T from MOG_35–55-induced EAE mice, as measured by quantitative RT-PCR, representative of n = 3 mice; ∗p ≤ 0.05 (unpaired, 2-tailed Student’s t test). b Representative histogram and average MFI (bar graph) of Runx3 and Eomes in EAE c-Met⁺ vs. c-Met⁻ CD3⁺CD8⁺ T cells, representative of n = 5 mice. c Eomes and Runx3 protein levels in spleen cells at peak disease (d14) c-Met⁺CD8⁺ vs. c-Met⁻ CD8⁺ T cells from MOG-induced EAE mice treated with either vehicle or HGF (30 ng/ml for 48 h) in the presence of IL-2; representative histograms are shown. Measurement of Eomes and Runx3 gMFI compared to isotype control of each condition (b, c). Data are representative of n = 5 mice. *p ≤ 0.05, **p ≤ 0.01 (unpaired, 2-tailed Student’s t test for b). Data from c are presented as mean ± SEM and were analyzed using the two-way ANOVA followed by Tukey post hoc comparisons. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001