Fig. 5
From: c-Met is expressed by highly autoreactive encephalitogenic CD8+ cells
HGF modulates the phenotype and function of c-Met+CD8+ T cells. a Cytolytic activity of CD8+ obtained from MOG35–55-induced EAE (score 2.5–3.5) stimulated ex vivo with MOG37–50 peptide for 48 h. Various effector/target (E/T) ratios were tested for killing of syngeneic B cells pulsed with MOG37–50 peptide (squares) or control gp10025–35 peptide (circles). Values represent the mean ± standard error of n = 3 mice per group, carried out in triplicate. Representative histogram and average MFI (bar graph) of granzyme B (b) and IFNγ (c) of c-Met+ vs. c-Met− CD3+CD8+ T cells from EAE mice (d14) treated ex vivo with HGF (30 ng/ml for 48 h) or untreated as vehicle; n = 5 mice/group. Data are presented as mean ± SEM and were analyzed using the two-way ANOVA followed by Tukey's post hoc comparisons. *p ≤ 0.05, **p ≤ 0.01