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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Functional relevance of the multi-drug transporter abcg2 on teriflunomide therapy in an animal model of multiple sclerosis

Fig. 1

Impact of abcg2-modulation on teri-induced effects in vitro. MACS sorted, activated splenic murine CD3+ T cells (α-CD3, 1 μg/mL; α-CD28, 10 ng/mL) from abcg2-KO and wt mice were treated with teri (12.5–100 μM) with or without ABCG2 inhibitor (FTC, 10 μM; Ko143, 10 μM). a Intracellular teri concentration after 2-6 h incubation; HPLC; LC-MS/MS; n = 4, MWU-test: *p < 0.05. b Proliferation index after 48 h incubation; CSFE, flow cytometry; n = 6-8, MWU-test: *p < 0.05; **p < 0.01; ***p < 0.001. c Apoptosis after 48 h incubation; Anx + PI, flow cytometry; n = 3, Wilcoxon test: *p < 0.05. wt: C57BL/6 J wild-type mice; abcg2-KO: abcg2-deficient mice on C57BL/6 J background. teri, teriflunomide; FTC, Fumitremorgin C

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