Skip to main content
Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Microglial depletion and repopulation in brain slice culture normalizes sensitized proinflammatory signaling

Fig. 1

Overall experimental design. a Experimental design for microglial depletion ex vivo. OHSCs were prepared as described below. On day 4 in vitro (4DIV), slices were treated with PLX3397 (1 μM) for 10 days to deplete microglia and then removed for analysis. b Protocol of microglial depletion and repopulation. OHSCs at 4DIV were treated with PLX3397 (1 μM) for 10 days and then returned to PLX-free medium for different time points as indicated. c Experimental design for microglial repopulation after chronic ethanol. OHSCs were treated with chronic binge ethanol (2 days on, 2 days off for 10 days) followed by microglial depletion and repopulation. Slices were analyzed after 14 days of repopulation

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094