Fig. 2

Microglial depletion by CSF1R inhibitor PLX3397 in primary ex vivo organotypic hippocampal slice culture (OHSC). a Quantification of microglial depletion in ex vivo OHSC by PLX3397A. Iba-1+ microglia count or Iba-1 mRNA in control and microglia depletion (M-Dep) groups. Representative Iba-1+ microglia immunostaining images are shown on the right. b Microglial marker gene expression was assessed by RT-PCR control and microglia depletion groups. c Gene expression of Toll-like receptors (TLRs) 2–9 following microglial depletion. *p < 0.05, **p < 0.01 vs control; n = 3 replicates/group