Table 2 Depletion of microglia reduces immune activation in response to ethanol. Brain slices were treated with vehicle or underwent microglial depletion with PLX3397 for 10 days, followed by ethanol (100 mM) treatment without PLX3397. Gene induction was assessed by RT-PCR after 4 days of ethanol treatment. Mean ± SEM

Cytokines

TNFα

100 ± 12

22 ± 4**

185 ± 16**

34 ± 5^††

IL-1β

100 ± 2

15 ± 2**

264 ± 17**

37 ± 1^††

IL-4

100 ± 14

239 ± 38*

34 ± 6*

139 ± 11^††

IL-6

100 ± 14

356 ± 20**

186 ± 50*

335 ± 6^††

IL-10

100 ± 4

151 ± 10*

217 ± 3**

224 ± 18^††

Toll-like receptors

TLR2

100 ± 0.4

56 ± 0.2**

184 ± 25**

68 ± 10^††

TLR3

100 ± 2

55 ± 6**

235 ± 3**

101 ± 2^††

TLR4

100 ± 3

104 ± 14

159 ± 1*

113 ± 6

TLR5

100 ± 5

16 ± 2**

112 ± 9

30 ± 2

TLR7

100 ± 9

23 ± 1**

195 ± 5**

49 ± 0.4^††

TLR9

100 ± 8

24 ± 1**

189 ± 5**

48 ± 9^††

Microglia markers

Iba-1

100 ± 0.3

21 ± 1**

149 ± 5*

45 ± 3^†

TREM2

100 ± 9

14 ± 0.5

76 ± 13*

32 ± 1^††

CD200R

100 ± 9

33 ± 1**

78 ± 4*

50 ± 5^††

CX3CR1

100 ± 3

12 ± 0.1**

69 ± 1*

116 ± 4

IL-4R

100 ± 10

37 ± 4**

144 ± 20*

66 ± 9^††

IRF8

100 ± 2

15 ± 0.1**

66 ± 3*

15 ± 1^††

Neuronal genes

mGluR2

100 ± 6

263 ± 56**

134 ± 11

158 ± 18^†

mGluR3

100 ± 9

170 ± 11*

92 ± 6

206 ± 24

mGluR5

100 ± 2

173 ± 23*

94 ± 21

167 ± 15^†

mGluR8

100 ± 10

213 ± 38**

169 ± 4*

364 ± 6^††

NR2A

100 ± 1

133 ± 0.5**

135 ± 5*

272 ± 7^††

NR2B

100 ± 5

137 ± 1*

123 ± 7

264 ± 2^††

PSD-95

100 ± 2

153 ± 21*

124 ± 1

201 ± 58^†

BDNF

100 ± 9

115 ± 6

205 ± 8**

550 ± 4^††

NGF

100 ± 3

115 ± 7

148 ± 21

162 ± 5*

NGFR

100 ± 3

52 ± 4*

76 ± 8

46 ± 0.2^††

CamK2a

100 ± 5

61 ± 4*

85 ± 3

104 ± 8

CamK2d

100 ± 9

137 ± 2*

117 ± 11

116 ± 10

Interferons

IFNα

100 ± 5

153 ± 5*

200 ± 23**

302 ± 1^††

IFNϒ

100 ± 9

160 ± 8**

39 ± 1**

94 ± 3