Table 3 Repopulation of microglia changes immune and neuronal gene expression. Brain slices were treated with vehicle or underwent microglial depletion with PLX3397 for 10 days, followed by repopulation for zero, 7 or 14 days. Gene induction was assessed by RT-PCR. Mean ± SEM

Cytokines

TNFα

100 ± 12

22 ± 4**

65 ± 6**

104 ± 8

IL-1β

100 ± 2

15 ± 2**

24 ± 1**

87 ± 6

IL-4

100 ± 14

211 ± 2**

303 ± 58**

267 ± 10**

IL-6

100 ± 14

93 ± 6

98 ± 1*

202 ± 5**

IL-10

100 ± 4

92 ± 6

163 ± 11*

436 ± 14**

Toll-like receptors

TLR2

100 ± 0.4

15 ± 2*

22 ± 1*

131 ± 16*

TLR3

100 ± 2

17 ± 0.5*

19 ± 1*

164 ± 9*

TLR4

100 ± 3

40 ± 4*

51 ± 1*

241 ± 19*

TLR5

100 ± 5

20 ± 1*

11 ± 2*

364 ± 28*

TLR7

100 ± 9

19 ± 1*

8 ± 0.3*

182 ± 6*

TLR9

100 ± 8

12 ± 1**

10 ± 0.3*

194 ± 11*

Microglia markers

Iba-1

100 ± 0.3

16 ± 1.3*

16 ± 0.4*

238 ± 49*

TREM2

100 ± 9

16 ± 1*

9 ± 0.3*

267 ± 11*

CD200R

100 ± 9

33 ± 1**

48 ± 4*

144 ± 13**

CX3CR1

100 ± 3

15 ± 2*

8 ± 0.2*

564 ± 18*

IL-4R

100 ± 10

41 ± 2*

98 ± 1*

202 ± 5*

IRF8

100 ± 2

16 ± 0.5*

8 ± 0.1**

160 ± 9*

CD68

102 ± 19

48 ± 1*

22 ± 0.2*

776 ± 94**

C1qA

100 ± 10

8 ± 1*

13 ± 0.4**

113 ± 4

C3

100 ± 12

6 ± 0.6*

7 ± 0.1**

47 ± 7**

Neuronal genes

mGluR2

100 ± 8

126 ± 10*

332 ± 26**

237 ± 31**

mGluR3

101 ± 4

146 ± 12*

142 ± 1*

289 ± 2**

mGluR5

100 ± 8

131 ± 4*

163 ± 9**

404 ± 34**

mGluR8

100 ± 4

227 ± 30*

176 ± 2**

677 ± 44**

NR2A

100 ± 3

143 ± 5*

165 ± 15*

212 ± 17**

NR2B

100 ± 5

127 ± 8*

173 ± 7*

304 ± 26**

PSD-95

100 ± 2

168 ± 11*

134 ± 12*

191 ± 18**

Synapsin

100 ± 4

152 ± 4*

116 ± 2

219 ± 14**

CX3CL1

100 ± 8

98 ± 2

79 ± 9*

295 ± 10**

Other genes

Arg-1

100 ± 3

165 ± 28*

144 ± 22*

1655 ± 328**

GFAP

100 ± 2

90 ± 1

133 ± 12*

148 ± 0.5*

GR

100 ± 3

97 ± 2

72 ± 5*

806 ± 28**

BDNF

100 ± 9

105 ± 6

135 ± 8*

265 ± 24**

NGF

100 ± 3

186 ± 9*

153 ± 10*

481 ± 5*