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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Deletion of SIRPα (signal regulatory protein-α) promotes phagocytic clearance of myelin debris in Wallerian degeneration, axon regeneration, and recovery from nerve injury

Fig. 2

SIRPα inhibits the phagocytosis of myelin debris in cultured BMDM (bone marrow-derived macrophages). a BMDM from wild-type (WT; +/+) but not SIRPα−/− (−/−) mice expressed SIRPα protein. Immunoblot of BMDM lysates using anti-SIRPα Ab determined the presence/absence of SIRPα protein (130KD). Immunoblot of same lysates using anti-α-tubulin Ab (55KD) verified equal protein load. b BMDM from SIRPα−/− mice (SIRPα−/−) phagocytosed more myelin debris than BMDM from wild-type mice (WT). BMDM plated at low cell density were exposed to myelin debris for 40 min and levels of phagocytosed myelin debris quantified by ELISA. Phagocytosis by SIRPα−/− BMDM is presented as percentage of phagocytosis by wild-type BMDM normalized to 100%. Box and whisker plot of eight experiments is given. The line represents the median, the box outlines the 25 to 75% range, and the whiskers extend to the highest and lowest observations. Double tailed value of significance, ****p < 0.0001, by unpaired t test

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Journal of Neuroinflammation

ISSN: 1742-2094