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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Deletion of SIRPα (signal regulatory protein-α) promotes phagocytic clearance of myelin debris in Wallerian degeneration, axon regeneration, and recovery from nerve injury

Fig. 3

The in vivo removal of myelin debris is faster in SIRPα−/− mice than in wild-type mice. Sciatic nerve segments undergoing Wallerian degeneration were removed from wild-type (WT) and SIRPα−/− mice at the indicated days after surgery, immediately lysed and protein content in lysates quantified. Levels of myelin-specific MBP (Myelin/MBP) in lysate samples of equal protein content were quantified using ELISA. Levels of Myelin/MBP in Wallerian degenerating nerve segments are presented as percentage of levels in intact nerves (time 0) normalized to 100%. Box and whisker plot of Myelin/MBP levels in five to ten different nerves is given. The line represents the median, the box outlines the 25 to 75% range, and whiskers extend to the highest and lowest observations. Significance of difference of WT mice from SIRPα−/− mice at the indicated days after surgery, ^p < 0.05 and ^^^p < 0.001, by two-way ANOVA and the Bonferroni multiple comparisons posttest. Significance of difference between levels of Myelin/MBP in intact nerves (day 0) and those at the indicated days after surgery, **p < 0.001 and ***p < 0.0001, by one-way ANOVA and the Dunnett posttest calculated for each mice strain separately

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Journal of Neuroinflammation

ISSN: 1742-2094