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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Rectification of radiotherapy-induced cognitive impairments in aged mice by reconstituted Sca-1+ stem cells from young donors

Fig. 4

Young Sca-1+ stem cells preserve synaptic connectivity and improve LTP in the CA1 hippocampus. a Representative image of dendritic spines from CA1 pyramidal neurons using Golgi-Cox staining for n = 4 mice per group. b Immunostaining for glutamatergic neurons in the CA1 hippocampus and dentate gyrus (top) and representative western-blot analysis for glutamate receptors (GluR1) from the whole hippocampus for at least n = 5 mice per group (bottom). c Field excitatory postsynaptic potential recordings and quantification from the CA1 hippocampus for at least n = 7 hippocampal slices per group. Scale bars, 5 μm (a), 200 μm and 100 μm for top and bottom images, respectively (b). Data are mean ± s.e.m. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001;†††† (after stimulation) or **** (relative to baseline before stimulation) P ≤ 0.0001 (two-way ANOVA with Tukey’s multiple comparisons test (b (left; F (1, 16) = 93.06, P < 0.0001)) and unpaired two-sided t-tests (a, b (right), c))

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