Fig. 1
Graphical illustration of the region sampled for ultrastructural and biochemical studies and age-related increases in C1q protein expression. a The region sampled for immunocytochemical, ultrastructure, and biochemistry studies. Schematic depicting the rhesus macaque brain and region (yellow oval) showing the location of the dorsolateral prefrontal cortex. The entire region was excised for biochemistry analyses to evaluate C1q protein expression. Approximately 60-μm-thick sections were cut for subsequent pre-embedding immunocytochemical labeling of C1q. b Cartoon illustrating the location of Walker’s area 46 and the principal sulcus (PS), which constitute the dlPFC and used for analyses in this study (highlighted in yellow). c, d Biochemical characterization of C1qA in macaque cortical tissue. Triton soluble macaque dlPFC tissue was immunoblotted for C1qA and GAPDH. Quantification of C1qA increase in macaque dlPFC with age was fit with an exponential growth curve (R2 = 0.5473). Each animal is color-coded by age