Fig. 6

Increased co-immunoprecipitation of C1q with PSD95 and gephyrin. a PSD95 (5 μg antibody) or gephyrin (10 μg antibody) was immunoprecipitated from 300 μg cortical tissue of the oldest macaque. The immunoprecipitated material was blotted for PSD95, gephyrin, and C1qA. b PSD95 was immunoprecipitated from the dlPFC of three animals along with a mouse IgG control (400 μg starting tissue in 8.3 and 25.3-year-old animals and 250 μg starting tissue in 27.1-year-old animal, mu IgG control was run on a pool of all three animal lysates ~ 250 μg). Immunoprecipitated elution was blotted for PSD95 and C1qA. c Quantification of C1qA levels co-immunoprecipitated with PSD95 represented as a ratio of C1qA over PSD95. The graph is color-coded by animal age as denoted in Fig. 1d