Fig 1

NK cell ameliorated EAMG symptoms and reduced serum anti-AChR97–116 IgG2a antibodies levels. NK cells were adoptively transferred into EAMG rats twice at the day before the first and second immunization, respectively. The body weights (a) and clinical scores (b) of NK cell-treated rats (n = 7) and PBS-treated rats (n = 6) were recorded every other day after the first immunization. The rats were sacrificed at the 46th day post the first immunization, and the blood sera were collected. Serum anti-AChR97–116 IgG, IgG1, IgG2a, and IgG2b antibody levels were determined by ELISA (c). Anti-AChR97–116 IgG antibody affinity was as determined by the thiocyanate method (d). Data were presented as mean ± SEM. Results were representatives of two independent experiments. Unpaired Student’s t test was used. Arrows mean intervention times. *p < 0.05, *p < 0.01, ***p < 0.001