Fig. 4

Phenotype differences between CXCR5^- and CXCR5⁺ NK cells. a Frequencies of CXCR5⁺ cell among blood, lymph node, and spleen CD3^-CD161^bright NK cells. Data were presented as mean ± SEM. One-way ANOVA. **p < 0.01. b Frequencies of IFN-γ⁺, IL-17⁺, ICOS⁺, and CD25⁺ cells within rat CXCR5^- and CXCR5⁺ NK cells. Data were presented as mean ± SEM. Results were representatives of two or three independent experiments. Unpaired Student’s t test was used. ns means not significant, *p < 0.05, **p < 0.01. c Representative flow cytometric histogram of the expression of CD27 on rat CXCR5^- and CXCR5⁺ NK cells (left). Mean fluorescent intensity (MFI) of CD27 expression of rat CXCR5^- and CXCR5⁺ NK cells (right). Data were presented as mean ± SEM. Three independent experiments. Unpaired Student’s t test was used. **p < 0.01. d Divide rat CXCR5^- and CXCR5⁺ NK cells into three subsets (CD27^low, CD27^median, and CD27^hi) according to CD27 expression levels (left). Frequencies of CD27^low, CD27^median, and CD27^hi cells among CXCR5^- and CXCR5⁺ NK cells (right). Three independent experiments. Data were presented as mean