Fig. 5

Distribution and migration of CXCR5⁺ and CXCR5^- NK cells. a Fluorescence image of a spleen from EAMG rat. Red indicated CD161, and green indicated CXCR5 (B cell zone). Arrowhead indicated CD161⁺CXCR5⁺ cells in the B cell zone. Scale bar, 100 μm. b Fluorescence image of EAMG rat lymph node. Red indicated CD161, and green (PNA) indicated germinal center area. Arrowhead indicated CD161⁺ cells in the germinal center area. Scale bar, 100 μm. c Some of NK cells migrated into B cell zone after adoptive transfer. NK cells, sorted by MACS sorting, were labeled with CFSE (green) and transferred into EAMG rats. Two days later, the spleen was harvested and stained with anti-IgM antibody (red). Arrowhead indicated NK cells in B cell zone, scale bar, 100 μm. d FACS sorted CXCR5⁺ and CXCR5^- NK cells were labeled with CFSE (green) and transferred into EAMG rats. Two days later, the spleen was harvested and stained with the anti-IgM antibody (red). Arrowhead indicated NK cells in B cell zone, scale bar, 100 μm