Fig. 2

Inhibition of Drp1-Fis1-mediated mitochondrial dysfunction is protective against LPS-mediated neuronal injury in culture. a Oxidative phosphorylation and glycolytic rate was measured with Seahorse Extracellular Flux in primary neurons were treated with 0.1 mg/ml LPS in the presence/absence of P110 (1 mM) for 24 h (n = 6). b Cellular ATP level was measured using CellTiter-Glo® after treatment as in a and represented as fold change of control. c Mitochondrial membrane potential was measured using TMRE after treatment as in a and represented as fold change of control (n = 6). d Drp1 levels and p53 levels were quantified by immunoblotting in enriched mitochondrial fractions and represented as fold change of control. VDAC was used as a loading control (n = 3). e LDH release was measured after treatment as in a and represented as % of Control (Con) (n = 6). Probability determined by one-way ANOVA and Holm-Sidak’s test for multiple comparisons between each treatment group, as above. All data are shown as the mean ± s.d., and p values are indicated