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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Mitochondrial dysfunction mediated through dynamin-related protein 1 (Drp1) propagates impairment in blood brain barrier in septic encephalopathy

Fig. 3

Inhibition of Drp1-Fis1-mediated mitochondrial dysfunction reduces oxidative stress in the brain and preserves endothelial integrity. a Balb/c mice were treated with LPS (8 mg/kg IP) to induce murine model of sepsis. Peptide P110 (0.5 mg/kg IP) was administered in a subset of septic animals 3 h following LPS treatment and tissue harvested at 24 h. b Hydrogen peroxide production was determined by Amplex RedTM from whole brain mitochondria after treatment as in a (n = 4). c ATP colorimetric assay kit was used to measure ATP levels in freshly isolated whole brain mitochondria after treatment as in a (n = 4). d ZO-1, occludin, VCAM-1, and ICAM-1 levels were quantified by immunoblotting. β-actin was used as a loading control (n = 4). e ZO-1, f occludin, g VCAM-1, and h ICAM-1 protein levels were quantified and represented as fold change (n = 4). Probability determined by one-way ANOVA and Holm-Sidak’s test for multiple comparisons between each treatment group, as above. All data are shown as the mean ± s.d., and p values are indicated

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