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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Mitochondrial dysfunction mediated through dynamin-related protein 1 (Drp1) propagates impairment in blood brain barrier in septic encephalopathy

Fig. 4

Inhibition of Drp1-Fis1-mediated mitochondrial dysfunction is protective in mouse model of septic encephalopathy. a Mitochondrial Drp1 translocation and cytochrome c release were quantified by immunoblotting in enriched mitochondrial fractions. VDAC was used as a loading control. b Drp1 and c cytochrome c protein levels were quantified and represented as fold change (n = 6). d TNFα, e IL-1β, and f IL-6 cytokine levels were measured in whole brain lysate by ELISA after a 24-h treatment of LPS (n = 6). g Weight changes in these mice were quantified as a surrogate for illness severity. (n = 5 mice for control, 5 for LPS-treated mice, and 5 for LPS + P110-treated). h Kaplan–Meier survival curve of mice showing increased survival following P110 treatment (red trace) as compared to the trans-activator of transcription (TAT)-treated control mice (black trace). Probability determined by one-way ANOVA and Holm-Sidak’s test for multiple comparisons between each treatment group, as above. All data are shown as the mean ± s.d., and p values are indicated

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