Fig. 1

NF-κB cellular localization in the AP of rats i.p. treated with TLR-4 agonists and antagonist LPS. Immunofluorescences of NF-κB in rat AP sections were performed. a The control group, b RS-LPS i.p. group, c PG-LPS i.p. group, and d EC-LPS i.p. group. In all cases, red corresponds to NF-κB immunosignal and blue DAPI staining in the nucleus. Arrows indicate red and blue immunosignal overlapping or NF-κB nuclear translocation. Head arrows indicate red immunosignal surrounding blue signal NF-κB cytoplasmic retention. Scale bars = 20 μm. e Quantitative analysis of the percentage of NF-κB nuclear translocation. f Quantitative analysis of the percentage of NF-κB cytoplasmic retention. Data (n = 4) were expressed as the mean (± SEM). *p < 0.05, ***p < 0.001, vs control. #p < 0.05, ###p < 0.001 vs RS-LPS. &p < 0.01, &&&p < 0.001 vs PG-LPS. One-way ANOVA with Tukey’s post hoc test