Fig. 12

Dectin-1, Syk, and p-Syk expression levels are significantly increased in LPS-induced BV2 cells. LAM/PIC pretreatment significantly decreased p-Syk, TNF-α, and iNOS expression in BV2 cells following LPS exposure. In addition, Dectin-1 expression was significantly decreased in the LPS-induced BV2 cells following LAM pretreatment. a, b The Dectin-1 and p-Syk expression levels were significantly higher at 3, 6, 12, and 24 h in the BV2 cells following LPS exposure compared with in the BV2 cells without LPS exposure (n = 3/group; ^*P < 0.05 vs. control group). Therefore, 24 h was selected as the optimal time point for BV2 cells exposed to LPS. c, d Dectin-1, p-Syk, TNF-α, and iNOS expression was significantly enhanced in BV2 cells with 24 h of LPS exposure compared with that of BV2 cells without LPS exposure. LAM pretreatment significantly decreased the expression levels of the aforementioned proteins in BV2 cells with 24 h of LPS exposure (n = 3/group; ^*P < 0.05 vs. control group; ^#P < 0.05 vs. LPS group). e, f BV2 cells with LPS exposure demonstrated significantly increased Dectin-1, p-Syk, TNF-α, and iNOS expression levels compared with that of BV2 cells without LPS exposure. PIC pretreatment significantly inhibited Syk, TNF-α, and iNOS expression in BV2 cells with LPS exposure compared with that of the LPS group (n = 3/group; ^*P < 0.05 vs. control group; ^#P < 0.05 vs. LPS group). However, PIC pretreatment failed to decrease Dectin-1 expression in BV2 cells with PIC pretreatment