Fig. 3

Increased hippocampal levels of IL-1β, IL-6, and NMDAR subunits after anesthesia and surgery were attenuated by MEM treatment on day 1 post-surgery. a Representative Western blots of IL-1β in the hippocampus. GAPDH was included as a loading control. b Quantitative analysis of IL-1β levels. c Representative Western blots of IL-6 in the hippocampus. GAPDH was included as loading control. d Quantitative analysis of IL-6 levels. e Representative Western blots of TNF-α in the hippocampus. GAPDH was included as loading control. f Quantitative analysis of TNF-α levels. g Representative Western blots of GluN2A. GAPDH was included as loading control. h Quantitative analysis of GluN2A levels. i Representative Western blots of GluN2B. GAPDH was included as loading control. j Quantitative analysis of GluN2B levels. Data are presented as the mean ± SEM (n = 6). *p < 0.05 compared to the con + veh group (**p < 0.01, ***p < 0.001), ^#p < 0.05 compared to the sur + veh group (^##p < 0.01, ^###p < 0.001)