Fig. 3

Effects of isotalatizidine on phosphorylation of p38, ERK1/2, and JNK in BV-2 and primary microglial cells. Effects of isotalatizidine on phosphorylation of p38, ERK1/2, and JNK in BV-2 (a) and primary microglial cells (b). Isotalatizidine (25 μM) obviously promoted the phosphorylation of p38 (c) and ERK1/2 (d), but not JNK (e) in cultured microglial cells. Selective p38 inhibitor SB 203580 (f, h) and ERK1/2 inhibitor U0126-EtOH (g, i) significantly blocked the isotalatizidine activated phosphorylation of p38 or ERK1/2, respectively. The data are expressed as mean ± SEM of three independent experiments. ^**P < 0.01, ^***P < 0.001 vs. control group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. isotalatizidine group