Fig. 4From: Isotalatizidine, a C19-diterpenoid alkaloid, attenuates chronic neuropathic pain through stimulating ERK/CREB signaling pathway-mediated microglial dynorphin A expressionIsotalatizidine stimulating the CREB activation and dynorphin A expression via ERK1/2 pathway in the primary microglial cells. In cultured primary microglial cells, isotalatizidine (25 μM) markedly induced the phosphorylation of CREB and expression of dynorphin A, and the phosphorylated levels of CREB and increased dynorphin A level were markedly prevented by ERK1/2 inhibitor of U0126-EtOH (a) but not p38 inhibitor of U0126-EtOH (b). Immunofluorescence analysis showed that isotalatizidine-stimulated IBA-1 expression and dynorphin A production also could be blocked by ERK1/2 inhibitor of SB203580 (c). The data are expressed as mean ± SEM (n = 3). *P < 0.05, **P < 0.01 vs. control group; #P < 0.05 vs. isotalatizidine groupBack to article page