Fig. 4

Clozapine treatment reduces CCL2- and CCL5-mediated migration. a In-vitro migration assay of bone marrow-derived macrophages treated with Latrunculin A or clozapine. b–i C57BL/6 female mice were treated with clozapine or vehicle control in their drinking water for 7 days. At the last day, CCL2 or CCL5 (left hind flank) or PBS (right hind flank) was injected s.c., and 18 h later, the draining LN cells were isolated and analyzed by flow cytometry. The total number of LN cells after CCL2 (b) or CCL5 injection (f) and of the individual cell types in the LN after CCL2 (c–e) or CCL5 injection (g–i) from three independent experiments (n = 13–14/group) are shown. P—PBS, 2—CCL2, 5—CCL5, *p < 0.0332 and **p < 0.021 by paired one-way ANOVA with Sidak’s multiple comparisons test within vehicle and clozapine treated groups. Comparison between CCL treatment in vehicle and clozapine mice was done by two-way ANOVA with Tukey’s multiple comparison test #p < 0.0332, ##p < 0.021, ###p < 0.0002, and ####p < 0.0001