Fig. 2

Changes in cell density in the dorsal SVZ and the adjacent corpus callosum at remyelination onset. a, d DAPI cell density, analyzed relative to the length (μm) and total area (μm²) of the dorsal SVZ. GFAP (b, e) and Iba1 (c, f) labelling in the dorsal SVZ supports the increase in the astrocyte (occupied area and intensity) and microglia (total number of microglia/mm²) cell density. g Morphological changes in microglia are evident in the dorsal SVZ but not in the adjacent demyelinated corpus callosum. h The intensity of DCX labelling in the dorsal SVZ and adjacent corpus callosum reconstructed by Imaris software. i DCX⁺ cell density in the dorsal SVZ showing no changes between the experimental groups. j Typical shape of a migrating DCX⁺ progenitor found in the corpus callosum and reconstructed by Imaris software. k, l Analysis of DCX⁺ cell density and the intensity of staining in the demyelinated corpus callosum. N = 6 mice per group. Statistics: *p ≤ 0.05; **p ≤ 0.01 vs. Sham; Student’s t test. Scale bar 10 μm in (j); 25 μm in (a, c); 50 μm in (b)