Fig. 4

Wnt gene expression are differentially regulated during corpus callosum remyelination and in microglia exposed to myelin. a, b Tissue dissection to evaluate Wnt3a, Wnt5a and Wnt7a gene expression in the demyelinated corpus callosum (day 35 p.i.), and during remyelination (day 42 p.i.) N = 6–10 mice per experimental group. Statistics: *p ≤ 0.05; **p ≤ 0.01 vs. Sham; #p ≤ 0.05; ##p ≤ 0.01 vs. TMEV day 35 p.i. (post-infection); ANOVA followed by Bonferroni’s post-hoc test. c, d In vitro analysis of Wnt5a and Wnt7a gene expression of microglia following the addition of purified myelin. N = 6 independent experiments with three replicates. Statistics: *p ≤ 0.05 vs. basal; Student’s t test