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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Involvement of Wnt7a in the role of M2c microglia in neural stem cell oligodendrogenesis

Fig. 5

Myelin contributes to the generation and maturation of oligodendrocytes from NSCs. a Scheme of the in vitro assays performed to evaluate the effects of purified myelin on the proliferation and differentiation of NSCs. b, c BrdU labelling and the proliferation of NSCs following the addition of purified myelin (1, 2.5, or 5 μm). d Triple immunocytochemistry performed after 2 days of NSCs differentiation to identify neurons (β-III Tubulin, red), astrocytes (GFAP, green), and oligodendrocytes (O4, yellow), as analyzed in (e). f O4 labelling performed after five div in the presence of purified myelin (1, 2.5, or 5 μm), and analyzed in (g) as the total area occupied by O4, and the proportion of OPCs, immature and mature oligodendrocytes. N = 3 independent experiments. Statistics: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 vs. Basal; ANOVA followed by Bonferroni’s post-hoc test. Scale bar: 50 μm

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