Fig. 7
From: Involvement of Wnt7a in the role of M2c microglia in neural stem cell oligodendrogenesis
M1 microglia secrete Wnt5a, while M2c microglia secrete the Wnt7a protein. a OX-42 staining of microglial cells following 24 h of stimulation with polarization agents to obtain the M1, M2a, and M2c activation states. b, d Wnt5a and Wnt7a gene expression by microglia in different activation states after 6 and 24 h of polarization. c, e Wnt5a and Wnt7a protein secreted after 24 h of polarization, evaluated in the supernatant of the cultures by ELISA. N = 4 independent experiments with three replicates. Statistics: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 vs. Control; ANOVA followed by Bonferroni’s post-hoc test. Scale bar: 25 μm