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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Involvement of Wnt7a in the role of M2c microglia in neural stem cell oligodendrogenesis

Fig. 8

M2c microglia conditioned medium contributes to the generation of oligodendrocytes from NSCs. (a) Scheme of the in vitro assays performed to evaluate the effects of conditioned media from control (MCM) and M2c (M2cCM) microglia on the proliferation and differentiation of NSCs. (b) b-Catenin and DAPI labelling following the addition of MCM or M2cCM to NSCs. (c, d) BrdU labelling and proportion of proliferative NSCs 1 div after the addition of MCM and M2cCM (1:1). (e) Triple immunocytochemistry performed after 2 days of NSC differentiation to identify neurons (b-III Tubulin, red), astrocytes (GFAP, green) and oligodendrocytes (O4, yellow), and analyzed in (f). (g) O4 labelling performed after 5 days of culture in the presence of MCM and M2cCM (1:1), and analyzed in (h) as the total area occupied by O4 and the proportion of OPCs, Immature and Mature oligodendrocytes. N = 3 independent experiments. Statistics: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 vs Basal; ANOVA followed by Bonferroni´s post-hoc test. Scale bar: 25 mm in a; 50 mm in c, e, g

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