Fig. 3

Myelin debris phagocytosis was reduced in MSR1 KO macrophages and increased in MSR1-overexpressed RAW264.7 cells in vitro. a Representative immunofluorescence images showing the engulfment of CFSE-labeled myelin debris (green) by different groups of macrophages (F4/80, red) after treatment with myelin debris. Scale bars = 20 μm. b Phagocytosis of myelin debris, as determined by counting the percentage of the CFSE+ area (n = 3 per group, values are the mean ± SD, **p < 0.01, two-tailed Student’s t tests). c Flow cytometric analysis of macrophages after treatment with CFSE-labeled myelin debris. The F4/80+ and CFSE+ quadrant represent myelin-laden macrophages. d Calculated percentages of F4/80+ and CFSE+ macrophages in different groups (MSR1 WT vs KO, MSR1 Vec vs OE) (n = 3 per group, values are the mean ± SD, ***p < 0.001, two-tailed Student’s t tests). e Oil Red O staining of foamy macrophages after treatment with myelin debris for 24 h. Scale bar = 20 μm. f Statistical analysis of the Oil Red O-stained areas in different groups of foamy macrophages (n = 3 per group, values are the mean ± SD, **p < 0.01, ***p < 0.001, two-tailed Student’s t tests)