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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Macrophage MSR1 promotes the formation of foamy macrophage and neuronal apoptosis after spinal cord injury

Fig. 6

MSR1 mediated activation of the NF-κB signaling pathway and the release of inflammatory factors after treatment with myelin debris. a, b Immunoblot images showing the effect of MSR1 knockout or overexpression on the expression of p-IκBα/IκBα with absence of myelin debris (n = 3 per group, values are the mean, NS indicates no significance, one-way ANOVA). c, d Altered protein expression level of p-IκBα/IκBα was detected using western blotting in MSR1 knockout or overexpression groups after treatment with myelin debris, or MSR1 WT macrophages and MSR1-overexpressed RAW264.7 cells treated with JSH-23 (an inhibitor of NF-κB), before treatment with myelin debris (n = 3 per group, values are the mean ± SD, *p < 0.05, **p < 0.01, one-way ANOVA, IN = JSH-23). e IF staining was used to analyze the distribution of p65 (green) in MSR1 WT and KO macrophages, and MSR1 Vec and OE RAW264.7 cells. The cell nuclei were stained with DAPI (blue fluorescence), scale bars = 20 μm. fg MSR1 WT and KO macrophages and MSR1 Vec and OE RAW264.7 cells were treated with myelin debris for 24 h, or MSR1 WT macrophages and MSR1 OE RAW264.7 cells treated with JSH-23 before adding myelin debris, and amount of secreted IL1-β and TNF-α in the supernatants of cell culture were detected by ELISA (n = 3 per group, values are the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA, IN = JSH-23)

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