Fig. 7

MSR1 promoted foamy macrophage-mediated neuronal apoptosis. a, b Representative photomicrographs of the Nissl-stained neurons in MSR1 WT and KO groups after SCI or sham surgery and the number of Nissl bodies was calculated (n = 3 per group, values are the mean ± SD, NS indicates no significance, **p < 0.01, one-way ANOVA, scale bars = 200 μm). c Representative images of neurons in neuronal cultures treated with the conditioned medium from different groups of macrophages and RAW 264.7 cells (n = 3 per group, MSR1 WT, MSR1 KO, MSR1 OE, MSR1 Vec, MSR1 WT + JSH-23, MSR1 OE + JSH-23) in the presence of myelin debris. Scale bar = 100 μm. d–e Sholl analysis of neurite growth in different groups (n = 3 per group, values are the mean ± SD, *p < 0.05, **p < 0.01, one-way ANOVA, IN = JSH-23). f, g Flow cytometric analysis of Neuronal apoptosis in different groups through Annexin V-FITC/PI double staining (n = 3 per group, values are the mean ± SD, **p < 0.01, ***p < 0.001, one-way ANOVA). h, i Western blot analysis of apoptosis-related proteins in neurons cultured in the conditioned media obtained from different groups. j, k Quantification of activated caspase-3 protein expression and the ratio of Bcl-2 to Bax in primary neurons cultured with the conditioned media obtained from different groups. Beta-actin was used as loading control (n = 3 per group, values are the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA)