Fig. 6

Effects of DMF on iNOS and COX-2 expression in NTG-induced migraine. The expression of iNOS and COX-2 was observed by Western blot analysis, in whole brain with the rostral cervical spinal cord samples of mice, 4 h after NTG-injections. A significant increase in iNOS expression was observed in NTG group (A), while DMF treatment significantly reduced iNOS expression at 30 and 100 mg/kg (A); F value = 18.92. Alike, COX-2 expression was significantly elevated in NTG-injected mice in comparison to controls (B). The rise in COX-2 expression induced by NTG was considerably blocked by treatment with DMF 30 and 100 mg/kg (B); F value = 37.47. Data are means ± SEM of ten mice for each group. A representative blot of lysates obtained from each group is shown and densitometry analysis of all animals is reported (n = 10 mice from each group). One-way ANOVA followed by Bonferroni post-test. **p < 0.01 and ***p < 0.001 vs. Sham; #p < 0.05, ##p < 0.01, and ###p < 0.001 vs. NTG; °p < 0.05 and °°p < 0.01 vs. Sham