Skip to main content
Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Systemic microbial TLR2 agonists induce neurodegeneration in Alzheimer’s disease mice

Fig. 6

TLR2 agonist increases microglial neurotoxic phenotype, but not inflammatory cytokines in 5xFAD microglia. a IBA1+ cell counts in cortical sections showed a basal 4-fold higher number of microglia in 5xFAD mice. Zymosan induced a marked increase in microglial number in wt mice but not in 5xFAD mice. b Real-time PCR for TLR2 expression in isolated CD11b + cells showed an 8-fold higher basal expression. Following TLR2 agonist stimulation there was 2-fold increased expression in wt, but 4-fold decreased TLR2 expression in 5xFAD microglia. c Real-time PCR showed mild differences in basal expression of inflammatory cytokines. d, e ICV injection of both low (5 μg, d) and medium dose (25 μg, e) zymosan induced similar cytokine expression in wt and 5xFAD microglia. f In vitro stimulation of microglia with zymosan caused significantly larger increase in ROS production in 5xFAD than in wt microglia. g In vitro stimulation of microglia with Zymosan caused a significant increase in NO production in 5xFAD but not in wt microglia

Back to article page