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Table 1 Antibodies used in immunofluorescent staining

From: Suppression of histone deacetylases by SAHA relieves bone cancer pain in rats via inhibiting activation of glial cells in spinal dorsal horn and dorsal root ganglia

  Antigens Primary antisera Secondary antisera
Single staining HDAC1
HDAC2
GFAP
Iba-1
Rabbit anti-HDAC1 IgG (1: 200; Sigma)
Rabbit anti-HDAC2 IgG (1:400; Cell Signaling Technology)
Mouse anti-GFAP IgG (1:5000; Chemicon)
Goat anti-Iba-1 IgG (1:800;
Abcam)
Alexa 488 donkey anti-rabbit IgG (1: 500) (Invitrogen)
Alexa 594 donkey anti-mouse IgG (1: 500) (Invitrogen)
Alex 594 donkey anti-goat IgG (1: 500) (Invitrogen)
Double staining HDAC1/NeuN
HDAC1/GFAP
HDAC1/Iba-1
HDAC2/NeuN
HDAC2/GFAP
HDAC2/Iba-1
Rabbit anti-HDAC1 IgG (1: 200; Sigma)
Rabbit anti-HDAC2 IgG (1:400; Cell Signaling Technology)
Mouse anti-NeuN IgG (1: 2000; Chemicon)
Mouse anti-GFAP IgG (1:5000; Chemicon)
Goat anti-Iba-1 IgG (1:800; Abcam)