Table 1 Antibodies used in immunofluorescent staining
| Â | Antigens | Primary antisera | Secondary antisera |
|---|---|---|---|
Single staining | HDAC1 HDAC2 GFAP Iba-1 | Rabbit anti-HDAC1 IgG (1: 200; Sigma) Rabbit anti-HDAC2 IgG (1:400; Cell Signaling Technology) Mouse anti-GFAP IgG (1:5000; Chemicon) Goat anti-Iba-1 IgG (1:800; Abcam) | Alexa 488 donkey anti-rabbit IgG (1: 500) (Invitrogen) Alexa 594 donkey anti-mouse IgG (1: 500) (Invitrogen) Alex 594 donkey anti-goat IgG (1: 500) (Invitrogen) |
Double staining | HDAC1/NeuN HDAC1/GFAP HDAC1/Iba-1 HDAC2/NeuN HDAC2/GFAP HDAC2/Iba-1 | Rabbit anti-HDAC1 IgG (1: 200; Sigma) Rabbit anti-HDAC2 IgG (1:400; Cell Signaling Technology) Mouse anti-NeuN IgG (1: 2000; Chemicon) Mouse anti-GFAP IgG (1:5000; Chemicon) Goat anti-Iba-1 IgG (1:800; Abcam) |