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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Influenza vaccination in early Alzheimer’s disease rescues amyloidosis and ameliorates cognitive deficits in APP/PS1 mice by inhibiting regulatory T cells

Fig. 4

IIV treatment enhanced microglial phagocytosis ability in APP/PS1 mice. a, f Representative merged microscopy images of Aβ plaques phagocytosed by microglia in AD and AD+IIV mice stained for microglia (Iba1, red), Aβ (Aβ, purple) and phagolysosomes (CD68, green). be, gj 3D reconstruction of red microglia (b, g), purple Aβ (c, h) and green phagolysosomes in microglia (colocalization of CD68 and Iba1, d, i) and merged images of transparent microglia and white amyloid plaques within microglial phagolysosomes (colocalization of Aβ plaques and microglial phagolysosomes, e, j) (scale bar, 15 μm). k Quantification of the percentages of Iba-1 immunostaining volume in close vicinity to Aβ plaques within the field in the AD and AD+IIV groups (n > 8, mean ± SEM, Student’s t test, **P < 0.01). l Assessment of the fractions of Aβ volume within microglial phagolysosomes, normalized to activated microglia, namely, the Aβ internalization ratio, revealed a threefold increase in Aβ internalization in the AD+IIV group compared with that in the AD group (n > 8, mean ± SEM, Student’s t test, **P < 0.01)

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