Fig. 8

The injection of rTMS stimulated ACM reduced neuronal apoptosis, infarct volumes, and promoted functional recovery in MCAO rats. Immediately after reperfusion and at 3 days post reperfusion, the MCAO rats received retroorbital intravenously injection of indicated ACM. At 7 days post reperfusion, apoptotic neurons were detected by NeuN/TUNEL (a), with scale bar of 50 μm. n = 3 animals per group. b Positive apoptotic neurons were calculated using ImageJ in at least five random fields. cRepresentative image of TTC staining in the indicated groups. n = 8 animals per group. d Quantitative analysis of infarct volume, n = 8. eThe neurological deficit was evaluated by modified Neurological Severity Score (mNSS). n = 8 animals per group. Data are represented as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 vs. sham rats. ^###P < 0.001 vs. MCAO + sham ACM group. ^$$$P < 0.001 vs. MCAO + O/R ACM group. O/R oxygen and glucose deprivation/reoxygenation