Fig. 2

Behavioral tests for limb strength and coordination. Grip strength change. a Shows grip strength changes of homotypic mice. b Shows grip strength changes of the four groups of mice. Data are presented as mean ± S.E.M. Latency of accelerating rotarod tests. c shows rotarod test latency changes of homotypic mice. d shows the rotarod test latency changes of the four groups of mice. Data are presented as mean ± S.E.M. Stride length tests change of mice. Data shown in (e) is presented as a boxplot with a median line in the box, a 75th percentile line on the top of box (Q3), a 25th percentile line at the bottom of the box (Q1), a maximum value bar calculated by Q3 + 1.5IQR (interquartile distance), a minimum value bar calculated by Q1–1.5IQR, and some abnormal values distant to the box. Dashed line denotes the median of male P301S mice stride length at the age of 3 months old. Composite phenotype scoring system test. f–j Shows the changes of composite phenotype total score, ledge test, clasp test, gait test, and kyphosis test, respectively, in all four groups. Statistical analysis was performed between sex-and age-matched P301S and WTs. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001