Fig. 1
Peli1 is increased after CCI and mainly expressed in spinal cord microglia. a The time course of Peli1 mRNA expression in the ipsilateral spinal cord in naive, sham, and CCI mice. CCI increased Peli1 mRNA levels at 3 days, 7 days, and 14 days (n = 6). One-way ANOVA (F4,25 = 18.0). b Quantitative western blot analysis showing the increase of Peli1 protein expression in the ipsilateral spinal cord on days 3, 7, and 14 after CCI (n = 6). One-way ANOVA (F4,25 = 22.8). c Representative images of Peli1 immunofluorescence in the L5 ipsilateral dorsal horn (scale 50 μm, n = 4). d Representative images of subcellular colocalization of Peli1 in the ipsilateral spinal dorsal horn with a high magnification image (boxed). Double immunofluorescence staining showing Peli1 (green) is primarily co-staining with microglial cells (Iba1, red), rarely with neurons (NeuN, red), and astrocytes (GFAP, red) (scale bar 50 μm, n = 4). Results are expressed as the Mean ± SEM. **p < 0.01, ***p < 0.001 compared with indicated group. ns statistically not significant